Selerity
Technologies Effectively Separates Active Ingredients in Natural
Products
Released on
= August 23, 2005, 1:02 am
Press Release
Author = Selerity Technologies
Industry =
Press Release
Summary = Selerity Technologies application of temperature programmed
liquid chromatography has resulted in improved efficiencies in separating
active components from natural products
Press Release
Body = The identification of both beneficial and potentially harmful
ingredients in natural products is under increased scrutiny, especially
in the food and nutraceutical industries.
The application of temperature programmed liquid chromatography
(TPLC) has resulted in improved efficiencies in separating active
components from natural products. In particular, research from Selerity
Technologies using the Series 8000 Oven has centered on the separation
of catechin and caffeine related compounds in cocoa,
green tea, and a commercially available ginseng tablet.
A new application
note is available free of charge from Selerity Technologies to describe
the improvements in separation time and peak shape by using thermal
gradients in place of a solvent gradient program.
Separation of
extracts from a number of natural products including cocoa, green
tea and ginseng has been conducted using Selerity Technologies Series
8000 Oven. A mixture of acidified water and methanol (80/20) was
used in all instances in the temperature programmed runs and all
runs were made under isocratic conditions.
Separations were obtained using a Selerity Technologies Blaze C8
column that withstands temperatures up to 100oC. In these separations,
Selerity Technologies did not attempt to identify all components.
The major compounds of interest in this group of products were the
catechins (catechin and epicatechin) and caffeine related compounds
(caffeine and theobromine).
The analysis
of cocoa is typically done using a solvent gradient of acidified
water and methanol. This separation consisted of a commercial cocoa
preparation (Hershey Company). The second experiment consisted of
a commercially available ginseng
tablet obtained from a nutritional store. Finally, the compounds
in a green tea preparation were separated. For each of these experiments,
the natural products tested could be conveniently separated using
a
thermal gradient in place of a solvent gradient program. The thermal
gradients resulted in better peak shape and method development is
rapid. The results were obtained after only two or three preliminary
runs.
Selerity’s
Series 8000 column oven with solvent preheater operates from ambient
to 250º C under either programmed or isothermal modes resulting
in faster run times and higher resolution separations. Polarity
and solubility changes make temperature programming a simpler option
for many gradient elution methods. The Blaze Column has been developed
by Selerity Technologies as part of a family of polycarbosilane
bonded silica columns that resist hydrolytic breakdown at temperatures
up to 200ºC under reverse phase conditions. Most silica-based
HPLC columns can only be used to temperatures up to 60°C and
a pH range of 3-8. Selerity's new Blaze 200 ™ column
overcomes this with a unique chemistry that protects the silica
backbone against hydrolytic attack, and creates better adherence
of the bonded phase.
For more information
about high temperature and temperature programmed liquid chromatography,
Blaze columns or Selerity’s range of TPLC instrumentation,
please visit www.selerity.com or e-mail sales@selerity.com . The
application note is available directly from Selerity Technologies,
please request application number
806.
– ENDS
–
For further
press information please contact: Wendy Bow, The Scott Partnership,
The Old Barn, Holly House Estate, CRANAGE, Middlewich, Cheshire
CW10 9LT, United Kingdom Tel: + 44 1606 837787 Fax: +44 1606 837757
mail to:pr@scottmail.co.uk
Web Site = www.selerity.com
Contact Details
= Selerity Technologies Inc
2484 West Custer Road
Salt Lake City, UT 84104
801-978-2295
801-978-2298
sales@selerity.com
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