Selerity Technologies Effectively Separates Active Ingredients in Natural Products

Released on = August 23, 2005, 1:02 am

Press Release Author = Selerity Technologies

Industry =

Press Release Summary = Selerity Technologies application of temperature programmed liquid chromatography has resulted in improved efficiencies in separating active components from natural products

Press Release Body = The identification of both beneficial and potentially harmful ingredients in natural products is under increased scrutiny, especially in the food and nutraceutical industries.
The application of temperature programmed liquid chromatography (TPLC) has resulted in improved efficiencies in separating active components from natural products. In particular, research from Selerity Technologies using the Series 8000 Oven has centered on the separation of catechin and caffeine related compounds in cocoa,
green tea, and a commercially available ginseng tablet.

A new application note is available free of charge from Selerity Technologies to describe the improvements in separation time and peak shape by using thermal gradients in place of a solvent gradient program.

Separation of extracts from a number of natural products including cocoa, green tea and ginseng has been conducted using Selerity Technologies Series 8000 Oven. A mixture of acidified water and methanol (80/20) was used in all instances in the temperature programmed runs and all runs were made under isocratic conditions.
Separations were obtained using a Selerity Technologies Blaze C8 column that withstands temperatures up to 100oC. In these separations, Selerity Technologies did not attempt to identify all components. The major compounds of interest in this group of products were the catechins (catechin and epicatechin) and caffeine related compounds (caffeine and theobromine).

The analysis of cocoa is typically done using a solvent gradient of acidified water and methanol. This separation consisted of a commercial cocoa preparation (Hershey Company). The second experiment consisted of a commercially available ginseng
tablet obtained from a nutritional store. Finally, the compounds in a green tea preparation were separated. For each of these experiments, the natural products tested could be conveniently separated using a
thermal gradient in place of a solvent gradient program. The thermal gradients resulted in better peak shape and method development is rapid. The results were obtained after only two or three preliminary runs.

Selerity’s Series 8000 column oven with solvent preheater operates from ambient to 250º C under either programmed or isothermal modes resulting in faster run times and higher resolution separations. Polarity and solubility changes make temperature programming a simpler option for many gradient elution methods. The Blaze Column has been developed by Selerity Technologies as part of a family of polycarbosilane bonded silica columns that resist hydrolytic breakdown at temperatures up to 200ºC under reverse phase conditions. Most silica-based HPLC columns can only be used to temperatures up to 60°C and a pH range of 3-8. Selerity's new Blaze 200 ™ column
overcomes this with a unique chemistry that protects the silica backbone against hydrolytic attack, and creates better adherence of the bonded phase.

For more information about high temperature and temperature programmed liquid chromatography, Blaze columns or Selerity’s range of TPLC instrumentation, please visit www.selerity.com or e-mail sales@selerity.com . The application note is available directly from Selerity Technologies, please request application number
806.

– ENDS –

For further press information please contact: Wendy Bow, The Scott Partnership, The Old Barn, Holly House Estate, CRANAGE, Middlewich, Cheshire CW10 9LT, United Kingdom Tel: + 44 1606 837787 Fax: +44 1606 837757 mail to:pr@scottmail.co.uk

Web Site = www.selerity.com

Contact Details = Selerity Technologies Inc
2484 West Custer Road
Salt Lake City, UT 84104
801-978-2295
801-978-2298
sales@selerity.com


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