Optimization of the Separation of Tocopherols Using Sub-Zero Temperature

Released on = June 23, 2005, 3:27 am

Press Release Author = Stephanie Marin

Industry =

Press Release Summary = Craft Technologies have used the Polaratherm from Selerity
Technologies to research whether the isocratic separation of tocopherols can be
enhanced using temperature programming.

Press Release Body = SALT LAKE CITY, Utah – Craft Technologies (Wilson, North
Carolina), has applied Selerity Technologies Polaratherm Total Temperature
Controller to its research into isocratic separation of tocopherols.
Previous separations were conducted at ambient temperature whereas this research
from Craft Technologies using sub-zero temperature demonstrates that the Polaratherm
offers a more effective alternative to traditional techniques. Polaratherm, the
first commercially viable product on the market capable of hot and cold temperatures
and temperature programming, overcomes the downfalls of conventional HPLC
separations conducted at ambient temperature resulting in improved resolution of
several isomers.

Vitamin E is a term used to describe the compounds contained in tocopherols.
In addition to their vitamin E activity, these compounds have been implicated in the
reduced risk of many diseases including cancer, cardiovascular disease, Alzheimer’s
and cataracts. Vitamin E also provides the greatest total antioxidant activity in
the lipoproteins in blood. No separation of all eight homologs of vitamin E has been
previously reported using isocratic reversed-phase HPLC.

The most commonly used method for the analysis of tocopherols is the direct
injection of the homologs into a C18 column using an ambient isocratic separation.
This results in the co elution of several isomers.
Using the Polaratherm, tocopherols can be effectively separated at -20°C.
The low temperature influences the column selectivity resulting into near-baseline
separation of tocopherols.

Selerity’s Polaratherm is a HPLC column compartment capable of controlling the
temperature from sub zero to 200ºC benefiting from two modes of operation,
temperature programming and isothermal. It is the first column compartment that
allows and regulates high, ambient, low or dynamic temperatures for selectivity
tuning and improved HPLC separations. This equipment is applicable to most vendor
HPLC systems and can be deployed in any laboratory already operating HPLC at little
additional cost.

For more information about the Polaratherm or Selerity’s range of TPLC
instrumentation, please visit www.selerity.com or e-mail sales@selerity.com.

Web Site = http://www.selerity.com

Contact Details = 2484 West Custer Road
Salt Lake City, UT 84104
Ph: 801-978-2295
Fax:801-978-2298
sales@selerity.com