Laminar Flow Cabinet

Pasir Panjang, Singapore, 2022-Jan-09 — /EPR Network/ — PCR Laminar Flow Cabinet is a type of ventilated enclosure with a vertical laminar airflow and provides product production for your PCR reagents and samples.

What is a laminar flow hood?
Clean benches and biological safety cabinets are common examples of laminar flow hoods. They are laboratory enclosures designed to carefully direct HEPA filtered air. Some of these hoods protect items placed on the work surface from contamination. Others prevent exposing the user to contaminants in the work area. Laminar flow hoods are often used to work with biological samples, semiconductors or other sensitive materials.

How is laminar air flow utilized in different types of equipment?

Class II Biosafety Cabinets, sometimes referred to as laminar flow hoods, maintain product protection through HEPA-filtered laminar downflow over the work zone. Per the NSF definition, these ventilated cabinets also feature inward airflow at the open front to protect operators and HEPA filtered exhaust air for environmental protection.

Class II, Type A cabinets recirculate air back into the laboratory unless a canopy connection is warranted.

Class II, Type B cabinets are hard-ducted to the outside.

Class II, Type C1 cabinets can function in either Type A or Type B mode. Whichever model suits your application, safe operation within biological safety cabinets is imperative to protect the integrity of your work and your personal safety.

PCR stations, enclosures that are specifically designed to house polymerase chain reaction experiment, utilize vertical flow of HEPA-filtered air to maintain a particulate-free work environment. A UV light is necessary to denature genetic material (DNA, RNA, etc.) and provide secondary decontamination.

Clean benches are suitable for applications that require product protection, such as media plate preparation or tissue culture maintenance. Air is drawn in through a prefilter located at the top of the clean bench before being pulled through a HEPA filter.

In a vertical clean bench, laminar air is then projected vertically over the work area. In a horizontal clean bench, laminar air is projected horizontally towards the operator. In both instances, laminar flow provides a particulate-free work area.

So, what is zoned airflow?
Zoned airflow is not truly laminar. Zoned airflow is used when equipment cannot achieve all of the protection required of a Class II biosafety cabinet with standard laminar airflow. Each zone, or column, of airflow is defined and has its own range in airspeed. This allows for higher speed barrier air columns to be utilized as an engineering solution to equipment that otherwise would have poor containment or product protection ratings.

How does laminar airflow differ from dilution flow?
Dilution flow is not the same as laminar air flow. The dilution flow principle is used in equipment such as filtered glove boxes. In these instances, HEPA-filtered air mixes with and dilutes interior airborne contaminants inside the glove box, and those contaminants are removed via a filtered exhaust system. After the contamination source has been sealed, the dilution rate—or air changes per minute—will determine how much time must lapse before materials can be removed from the main chamber.